Neurogenesis- Creation of Cortical Neurons from mESCs
This was part of a project I did at UoH. I'm not even sure if I should be putting this up here, tbh. It's only for the good I guess ;) Abstract Production of cortical neurons is a fairly extensive yet beautiful procedure starting from mESCs to NPCs to neurons. The neuronal precursors and neurons thus harvested can be used to study epigenetic regulations characteristic of and involved in the differentiation of neurons and related cells. In the followed protocol, a defined medium with no morphogen has been used. Use of morphogen has been replaced by an Shh inhibitor cyclopamine; thus, ensuring the differentiation of ESCs into cortical neurons only. When NPCs and neurons are harvested at day 14 and day 21 respectively, several other kinds of cells may be obtained too which are discarded off via FACS (Fluorescence Activated Cell Sorting). Also, no feeder cells are being used. Instead, a coating of gelatin (until day 12) and poly-L-lysine/laminin (from day 12) takes up the ...